本书是为分子生物学双语教学而编写，适用于高年级本科学生使用。本书内容涵盖分子生物学的中心问题，即DNA复制、转录、翻译和重组。我们从该领域的很基本内容（生物大分子的结构与功能）开始，系统、完整地阐述了上述主题，并引领读者贯通现代知识体系，从而深入理解这些过程是如何进行调控并创造出我们称之为“生命”的动态系统的。我们有目的地使用简洁的语言和详尽的插图来强化双语教学。书中通过嵌入二维码的方式配备了全文各章节英语课文、单词表音频录音，以及英汉对照名词解释，由此进一步强化双语教学目标。二维码内容还包含一些重要的实验技术双语内容，可以帮助学生将课堂所学分子生物学知识与课外学习活动联系起来。通过提供关于分子生物学重要内容和相关领域专业英语方面的训练，本教材能真正为读者提供一种现代的、切合实际的学习方式。
Introduction
Chapter 1 Amino Acids to Proteins
1.1Protein Composition
1.2Protein Conformations
1.2.1Describing Protein Structure
1.2.2Chemical and Physical Basis for Protein Folding
1.3Protein Structure and Function:A Few Examples
1.4The Dynamics of Proteins
1.5Experiments
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 2 Nucleic Acids
2.1Properties of a Genetic Material
2.2Nucleic Acids and DNA structure
2.2.1Nucleotides
2.2.2General Structure of Nucleic Acids
2.2.3Structure of DNA
2.3DNA as the Genetic Material
2.4DNA in the Cell
2.5RNA (Ribonucleic Acid)
2.6Experiments
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 3 Transcription in Prokaryotes:Mechanism and Regulation
3.1Why Use an RNA Intermediate
3.2Mechanism of Transcription
3.2.1Promoters
3.2.2RNA Polymerase
3.2.3Transcription Mechanism in Three Phases
3.3Regulation of Gene Expression in Prokaryotes
3.3.1Coordinate Regulation
3.3.2The Lac Operon
3.3.3The Trp Operon
3.3.4Ara and Gal Operons
3.4Experiments
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 4 Transcription in Eukaryotes:Mechanism and Regulation
4.1Eukaryotic RNA Polymerases
4.2Eukaryotic Promoters
4.3General Transcription Factors and Initiation
4.3.1TFⅡD
4.3.2Other TFⅡs
4.3.3General Transcription Factors for RNA Polymerase Ⅰand Ⅲ
4.4Specific Transcription Factors and Transcriptional Regulation
4.4.1Activators
4.4.2Repressors
4.4.3Enhancers and Silencers
4.5Structures of Specific Transcription Factors
4.5.1DNA-Binding Motifs in Prokaryotes
4.5.2DNA-Binding Motifs in Eukaryotes
4.6Experiments
4.6.1RNA Polymerase Targets
4.6.2Modularity of Specific Transcription Factors
Vocabulary
Review Questions
Exploration Questions
Chapter 5 mRNA Modifications in Eukaryotes
5.1Capping
5.2Polyadenylation
5.3Splicing
5.3.1The Basic Splicing Reaction
5.3.2Proteins Involved in Splicing
5.3.3Self-Splicing
5.3.4Trans-Splicing
5.3.5Reasons for Introns
5.4mRNA Editing
5.5Experiments
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 6 Translation
6.1The Genetic Code
6.2Mechanism of Translation in Prokaryotes
6.2.1Initiation
6.2.2Elongation
6.2.3Termination
6.3Translation in Eukaryotes
6.4tRNA Structure and Wobble
6.4.1Anti-codons
6.4.2Wobble
6.5Experiments
6.5.1Deciphering the Genetic Code
6.5.2Direction of Translation
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 7 Regulation of Gene Expression in Eukaryotes
7.1Histones and Transcriptional Regulation
7.1.1Histones and DNA Organization
7.1.2Histones and Transcription
7.1.3Covalent Modification of Histones
7.1.4Proteins that Recognize and Modify Histones
7.2Post-Transcriptional Regulation
7.3Nuclear Export
7.4RNA Stability
7.4.1mRNA Stability Regulation by Proteins
7.4.2mRNA Stability Regulation by Small RNAs
7.5Translational Control
7.5.1Global Control
7.5.2mRNA-Specific Control
7.6mRNA Localization
7.7Protein Regulation
7.8Experiments
7.8.1Beads-on-a-string Structure
7.8.2Repression of Gene Expression in Heterochromatin
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 8 DNA Replication
8.1Semi-Conservative Replication
8.2Initiation of Replication
8.3Semi-Discontinuous Replication
8.4Elongation of Replication and its Proteins
8.4.1Helicase and SSBs
8.4.2DNA Polymerases
8.4.3Explanation for 3′→5′Synthesis
8.4.4Primers
8.5DNA Topology
8.6DNA Replication in Eukaryotes
8.6.1Initiation of DNA Replication in Eukaryotes
8.6.2Telomeres
8.7Experiments
Summary
Vocabulary
Review Questions
Exploration Questions
Chapter 9 Mutations and Mutation Repair
9.1DNA Damage and Mutations
9.2Point Mutations
9.2.1Mismatched Base
9.2.2Spontaneous Mutations
9.2.3Induced Mutations
9.3Insertions and Deletions
9.3.1Strand Slippage
9.3.2Transposons
9.3.3Intercalating Agents
9.4Large-Scale DNA Changes
9.5Consequences of DNA Mutations
9.5.1Consequences of Point Mutations
9.5.2Consequences of Insertions and Deletions
9.5.3Consequences of Translocations
9.5.4Mutation Hot Spots
9.6Mutation Repair
9.6.1Direct Reversal
9.6.2Mismatch Repair
9.6.3Nucleotide Excision Repai
王勇，江苏大学食品与生物工程学院，副教授，王勇，江苏大学食品与生物工程学院，副教授。1985年毕业于复旦大学生物系，获理学学士学位；2003年毕业于江苏大学生命科学研究院，获工学博士学位。1985-1997年就职于中国农业科学院蚕业研究所（镇江），1994-1996年主持完成了国家自然科学基金“人工合成抗菌肽基因导入桑树的研究”课题；1997-2001年就职于中美合作上海实宏纸业有限公司；2001年以后在江苏大学任教，讲授硕士研究生《分子生物学》双语课程，本科《分子生物学》双语课程，本科《生物信息学》双语课程。2006年与美国学者Sylvain W. Lapan先生合作完成《英汉对照分子生物学导论》讲义稿，用于2006~2007年本科教学总结、完善后，于2008年送交化学工业出版社正式出版。
所获奖项：江苏大学2015年度本科教学优选个人、“分子生物学双语课程建设与实践”获江苏大学高等教育教学成果一等奖（2009年）、“分子生物学双语教学实践与探索”获全国首届生物化学与分子生物学教学研讨会论文三等奖（2007年）。
